Blog Posts

Polyglutamine research funded by NIH

Starting January 2015, the NIH NIGMS has awarded our R01 proposal on the molecular characterization of the misfolding and aggregation of expanded polyglutamine proteins. Polyglutamine expansion underlies various related diseaseas, including Huntington’s Disease and several other ataxias. The nature of the misfolding mechanism and the structures of the misfolded species remain poorly understood. The R01 will support our ongoing studies using magic-angle-spinning NMR (and other tools) to provide the need structural insights.

Congratulations to Dr Cody Hoop!

Congratulations to Cody, for defending her PhD thesis entitled “Site-specific structure and dynamics of polyglutamine-containing amyloid fibrils and the caveolin scaffolding domain by magic angle spinning solid-state NMR.”!

Publication – Hoop et al (2014) “Polyglutamine amyloid core boundaries and flanking domain dynamics in huntingtin fragment fibrils determined by solid-state NMR.”

New publication on the structure and dynamics in fibrils formed from mutant huntingtin fragments, including a 44-Q exon-1. In the journal Biochemistry: Polyglutamine amyloid core boundaries and flanking domain dynamics in huntingtin fragment fibrils determined by solid-state NMR. DOI:http://dx.doi.org/10.1021/bi501010q

Publication – D-polyglutamine amyloid recruits L-polyglutamine monomers and kills cells.

New publication on the surprising features of D-polyQ, in the Journal of Molecular Biology: D-polyglutamine amyloid recruits L-polyglutamine monomers and kills cells. DOI:http://dx.doi.org/10.1016/j.jmb.2013.11.019

Registration extended: upcoming continuing education tour of the structural biology dept facilities

Event on Oct 12th. Registration deadline: Oct 8th. Organized by the Spectroscopy Society of Pittsburgh:

http://www.ssp-pgh.org/wp-content/uploads/2013/09/SSP-Flyer-Structural-Biology-Tour_2.pdf

 

 

Publication – Spinning-rate encoded chemical shift correlations from rotational resonance solid-state NMR experiments

Jun Li’s work on rotational resonance width experiments is published in JMR:

Li, J., & van der Wel, P.C.A. Spinning-rate encoded chemical shift correlations from rotational resonance solid-state NMR experiments. Journal of Magnetic Resonance. 2013;230:117-124.  http://dx.doi.org/10.1016/j.jmr.2013.02.004

Publication – β-hairpin-mediated nucleation of polyglutamine amyloid formation

Cody’s collaborative work with the Wetzel group on the structure and formation mechanism of polyglutamine aggregates is published in JMB:

Kar, K., Hoop, C.L., Drombosky, K.W., Baker, M.A., Kodali, R., Arduini, I., van der Wel, P.C.A., Horne, W.S., & Wetzel, R. β-hairpin-mediated nucleation of polyglutamine amyloid formation. J Mol Biol. 2013;425(7):1-45.

See:  http://dx.doi.org/10.1016/j.jmb.2013.01.016

MBSB T32 fellowship awarded to Cody!

The Pitt/CMU Molecular Biophysics & Structural Biology graduate program has awarded one of its three T32 fellowships to Cody. Congratulations and great job!

New website

New wordpress-based website is now (more or less) functional.