Tag: Barth syndrome

Publication: Protocols for membrane protein studies by solid-state NMR (including drug interactions)

Our invited ‘protocol’ paper is now available in the journal Protein Science (Protein Society), authored by Alessia Lasorsa and Patrick van der Wel. In this report we described our “Solid-state NMR protocols for unveiling dynamics and (drug) interactions of membrane-bound proteins”. This paper was based on an invitation to contribute to the “Tools for Protein Science 2025” special issue. We opted for contributing a discussion of how we use solid-state NMR to probe membrane-bound proteins. More specifically, we summarized protocols we developed in years of work on mitochondrial lipid-protein interactions. A special focus in the report is on some recent work with the group of Valerian Kagan (at journal) where we probed how drug-like compounds can bind a pathogenic protein-lipid complex relevant to Barth Syndrome. We hope that the resulting manuscript is a useful reference work for people working on these topics, and in particular also for introducing new ssNMR users to relevant procedures (and their rationales). 

We acknowledge the funding support of the Barth Syndrome Foundation, who is currently supporting the related work in the lab.

Reference:

Lasorsa, A., Van der Wel, P.C.A. (2025) Solid-state NMR protocols for unveiling dynamics and (drug) interactions of membrane-bound proteins. Protein Science 34: e70102. [DOI]